MiR-370 functions as oncogene in melanoma by direct targeting pyruvate dehydrogenase B

Abstract Background Accumulating evidence has shown that miR-370 play an important role in the development and progression of tumor. However, the role of miR-370 in melanoma remains largely unknown. The present study is designed to investigate the function of miR-370 in melanoma and to explore the molecular mechanism underlying its function. Materials and methods The expression level of miR-370 was detected in melanoma tissues and cell lines by real-time quantitative polymerase chain reaction (qRT-PCR). The effect of overexpression of miR-370 on in vitro cell proliferation, apoptosis, invasion as well as glyclolysis was examined. Western blotting analysis was used to detect the influence of miR-370 on the expression of target genes, and Pearson analysis was used to calculate the correlation between the expression of targets gene and miR-370 in melanoma tissues. Results Our study showed that miR-370 was upregulated in melanoma tissues compared with non-cancerous tissues (P  Conclusions In conclusion, our findings suggested that miR-370 represents a potential oncogenic miRNA and plays an important role in melanoma progression by directly targeting PDHB.