Antibacterial and Antifungal Activities of Green Alga Cladophora Crispata

Algae contains antimicrobial substances, these substsances have inhibitory effects on pathogenic mi- crobes. In present study extracts of green alga Cladophora crispata (Roth.) Kuetzing were prepared in different solvent and tested against bacterial & fungal strains. Antibacterial and antifungal activity of algal extracts were assayed by agar well diffusion method and by agar plug method respectively. Bacterial strains such as Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa and Salmonella typhi were selected. Fungal strains which were selected for antifungal assay were Metarhizium anisopliae , Curvularia lunata, Rhizoctonia solani, Penicillium oxalicum, Aspergillus niger, Trichoderma viridae and Fusarium oxysporum. Extracts prepared in acetone ,chloroform, petroleum ether and toluene shows notable antibacterial activity against selected bacterial strains. The zone of inhibition ranges from 4mm to 15mm. In antifungal assay algal extracts prepared in ethanol, Methanol, acetone and chloroform shows antifungal activity. In antifungal assay overall the zone of inhibition ranges from 4mm to 15mm. Cold water and hot water ex- tracts also shows antifungal activity. In antifungal assay overall the zone of inhibition ranges from 4mm to19mm..Algal extract with toluene shows very high antimicrobial activities as compared with other.