The Use of Synthetic Oligo-RNA and -DNA as Defined Templates for the Determination Of Catalytic Properties of RNA-Directed RNA Polymerase from Tomato Leaf Tissue

Abstract The in vitro transcription of nucleic acids by RNA-directed RNA polymerase, purified from tomato leaf tissue and obtained as a single polypeptide of electrophoretic homogeneity, was studied using defined oligo-RNA and -DNA as template. From the analysis of transcription products certain novel catalytic properties of RdRP became apparent.