Trigger of central nervous system oxygen toxicity by a caffeine-containing vitamin functional beverage in mice

Objective To investigate the triggering effect of a caffeine-containing vitamin functional beverage on central nervous system oxygen toxicity (CNS-OT) in mice. Methods Forty SPF mice were randomly divided into the blank group, the control group, the placebo group and the caffeine-containing vitamin functional beverage group (or the experimental group), each consisting of 10 animals. The animals in the blank and control groups were left there intact, while the animals in the placebo group were given 0.4 ml physiological saline by gavage and those in the experimental group were given caffeine-containing vitamin functional beverage also by gavage, 30 min after gavage, the CNS-OT model was developed. Effects were evaluated through the approaches of seizure latency, convulsion frequency and malondialdehyde (MDA) index in the brain tissue. Results Following application of the caffeine-containing vitamin functional beverage, CNS-OT seizure latency was obviously shortened, as compared with that of the control group (P<0.01). In the process of 30 min exposure, there was no significant changes in oxygen convulsion frequency, and there was a slight increase in the MDA index in the brain tissue after HBO exposure, but without statistical significance (P<0.01). Conclusion The caffeine-containing vitamin functional beverage could significantly shorten CNS-OT latency and promote oxygen convulsion. Key words: Central nervous system oxygen toxicity; Caffeine; Malondialdehyde; Oxygen convulsion