Additional file 2: Figure S1. of Booster vaccination against tetanus and diphtheria: insufficient protection against diphtheria in young and elderly adults

Gating strategy for the analysis of the intracellular stainings. A representative example of CD4+ memory T cells producing IFN-γ, TNF-α, IL-2, IL-4, IL-10, IL-17, IL-21, TGF-β and GM-CSF after 6 h of tetanus toxoid (10 μg/ml) stimulation is shown (TIF 865 kb)