Marinobacter vinifirmus sp. nov., a moderately halophilic bacterium isolated from a wine-barrel-decalcification wastewater.

A halophilic, Gram-negative, motile, non-sporulating bacterium designated strain FB1T was isolated from a wine-barrel-decalcification wastewater. The organism comprises straight rods and has a strictly respiratory metabolism with O2. Strain FB1T grows optimally at 20–30 °C and 5–6 % NaCl. The predominant fatty acids were found to be C18 : 1 ω9c (30.4 %), C16 : 0 (25.7 %), C12 : 0 3-OH (10.3 %), C16 : 1 ω9c (9.7 %) and C16 : 1 ω7c (8.4 %). A phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain forms a coherent cluster within the genus Marinobacter. The highest level of 16S rRNA gene sequence similarity (97.9 %) exhibited by strain FB1T was with the type strain of Marinobacter excellens. However, the level of DNA–DNA relatedness between the novel strain and M. excellens CIP 107686T was only 31.2 %. The DNA G+C content of strain FB1T was 58.7 mol%. On the basis of phenotypic and genotypic characteristics, and also phylogenetic evidence, strain FB1T is considered to represent a novel species of the genus Marinobacter, for which the name Marinobacter vinifirmus sp. nov. is proposed. The type strain is FB1T (=DSM 17747T=CCUG 52119T).