Synthesis and anti-leishmanial activity of 5-(5-nitrofuran-2-yl)-1,3,4-thiadiazol-2-amines containing N-[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl] moieties

Abstract A novel series of 5-(5-nitrofuran-2-yl)-1,3,4-thiadiazol-2-amines were synthesized by introducing N -[(1-benzyl-1 H -1,2,3-triazol-4-yl)methyl] moiety as a new functionality on the C-2 amine of thiadiazole ring via click chemistry. The title compounds namely, N -[(1-benzyl-1 H -1,2,3-triazol-4-yl)methyl]-5-(5-nitrofuran-2-yl)-1,3,4-thiadiazol-2-amines ( 3a – n ) were characterized by IR, NMR and MS spectra. These compounds were evaluated for their in vitro anti-leishmanial activity against promostigote form of the Leishmania major . Most compounds exhibited good anti-leishmanial activity against the promastigote form of L. major . The most active compound against promostigotes was found to be 4-methylbenzyl analog 3i , which significantly decreases the number of intracellular amastigotes per macrophage, percentage of macrophage infectivity and infectivity index.