Relationship between concomitant injuries sustained during acl rupture and biological markers of articular cartilage metabolism

s / Osteoarthritis and Cartilage 22 (2014) S57–S489 S70 had a majority of ARGS fragments (70, 61 and 77%, respectively), while the reference and the JIA groups had high proportion of CS2-G3 fragments (53 and 52%, respectively). Estimates of the relative mol-mol proportions of the pathological aggrecanase cleavage in the IGD generating ARGS-fragment and the aggrecanase turnover cut in the CS2 region generating CS2-G3 fragments showed that the ARGS proportion was 27% for the reference group while it was between 69 and 82% in the adult and juvenile injury groups and in OA group. A similar comparison of MMP generated FFGV (a cut in the IGD of aggrecan) and the aggrecanase generated ARGS fragments showed that the FFGV fragments amounted to 32% for the reference group while the proportion was much lower for the adult injury (2.1%), juvenile injury (7.3%) and the OA (1.2%) groups. Fig 1. Proportion (mol/mol) of full length aggrecan (G1-G3), aggrecanase generated ARGS and CS2-G3 fragments, MMP generated FFGV fragments and calpain generated IGD-PGVA fragments found in synovial fluid pools of different patient Groups. Conclusions: The OA, juvenile and adult knee injury groups show similar aggrecan fragmentation patterns, which differ from the fragmentation pattern of JIA and knee healthy reference groups. This suggests that the aggrecan fragmentation patterns are different between different joint diseases. This information supports further understanding of mechanisms of cartilage damage in these conditions, and may aid to distinguish different patient groups. 109 RELATIONSHIP BETWEEN CONCOMITANT INJURIES SUSTAINED DURING ACL RUPTURE AND BIOLOGICAL MARKERS OF ARTICULAR CARTILAGE METABOLISM S.M. Wasilko y, T.W. Tourville y, M.J. DeSarno y, J.R. Slauterbeck y, R.J. Johnson y, A. Struglics z, B.D. Beynnon y. yUniv. of Vermont Coll. of Med., Burlington, VT, USA; z Lund Univ., Dept. of Orthopaedics, Clinical