New application of protein derived from clover and its coding genes
2013
The invention discloses a new application of protein derived from clover and its coding genes. The new application provided in the invention is a method for cultivating transgenic nodulation plants with high nodulation capability and/or high stress tolerance by using MtWRKY76 coding nucleic acid molecules. The method for cultivating transgenic nodulation plants with high nodulation capability and/or high stress tolerance provided by the invention comprises the step of introducing the MtWRKY76 gene into a receptor nodulation plant to obtain a transgenic nodulation plant with nodulation capability and/or stress tolerance higher than those of the receptor nodulation plant; the MtWRKY76 is protein as shown in the following a) or b), wherein a) protein with an amino acid sequence as shown in SEQ ID No.2; b) protein which is obtained by substitution and/or deletion and/or addition of one or more than one amino acid residue in SEQ ID No.2, is related to the nodulation capability and/or stress tolerance of nodulation plants, and is derived from a). The new application of the invention has significant value in cultivation of transgenic leguminous crops with salt resistance, drought tolerance, and enhanced nodulation capability.
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