Sex Differences in Muscle Metaboreflex Activation following Static Handgrip Exercise.

Purpose Larger blood pressure (BP) responses to relative-intensity static exercise in males vs. females is thought to involve altered muscle metaboreflex activation, but whether this is due to an intrinsic sex difference in metabolite production or to differences in muscle strength and absolute load is unknown. Methods Continuous BP and heart rate were recorded in 200 healthy young males and females (females: n = 109) during 2 minutes of static handgrip exercise at 30% of maximal voluntary contraction (MVC), followed by 2 minutes of post-exercise circulatory occlusion (PECO). Muscle sympathetic nerve activity (MSNA) was recorded in a subset of participants (n = 39; female n = 21), permitting calculation of signal-averaged resting sympathetic transduction (MSNA-diastolic BP). Sex differences were examined with and without statistical adjustment for MVC. Multivariate regression analyses were performed to identify predictors of BP responses. Results Males had larger systolic BP responses (interactions, P 0.7). In the subset with MSNA, neither burst frequency or incidence responses to static handgrip exercise or PECO differed between males and females (interactions, P > 0.2). Resting sympathetic transduction was also similar (P = 0.8). Multivariate analysis showed that MVC, the change in MSNA, and sympathetic transduction were predictors of the systolic BP response to static handgrip but only MVC was associated with responses during PECO. Conclusion Sex differences in absolute contraction load contribute to differences in BP responses during muscle metaboreflex isolation using PECO. These data do not support an intrinsic effect of sex as being responsible for exercise BP differences between males and females.