Express the recombinant protein PD-L1 in prokaryotic and analyze its biological activity SHEN Li-ping,

Objective To construct the programmed cell death 1 ligant 1 (PD-L1)recombination expression vector,express the fusion protein in prokaryotic and analyze the biological action of express product. Methods The whole PD-L1 gene sequence was synthesized after codon optimized. Construct the thioredoxin-(PD-L1) recombination expression vector and express the fusion protein in E. coli. Purified the target protein and analyze the conjugated ability of protein by ELISA. Results The PD-L1 recombinant expression vector has been constructed correctly. The target protein has been obtained with which expressed in high efficiency and production. The target protein can conjugate specifically with the PD-1, its specific receptor. Conclusion We have obtained the PD-L1 recombinant protein success with high biological activity. The result provide the basic condition for further study on antibody and mutually action between PD-L1 and chronic virus infectious. Key words: Codon; Cell death; Recombination proteins; Thioredoxin