Method for detecting and correcting sequential data error in sequencing result

The invention provides a method for detecting and correcting a sequential data error in a sequencing result. At least two nucleotide substrate molecules of different basic groups are contained in a sequencing reaction solution; and simplified gene coding messages are acquired. Two or more than two simplified gene coding messages are contrasted with each other, and persons skilled in the art can judge whether the condition of mutual contradiction appears in the coding of the position. Correction is carried out by using the method provided by the invention, micro improvement on original sequencing data error rate can be reduced for any sequencing method, and the error rate of coding data after the correction can be obviously reduced.