Establishment of a Suspension Cell System for Transformation of Jatropha curcas Using Nanoparticles

Cell suspension cultures of Jatropha curcas were established and optimized in shake flasks. The stem segments of Jatropha curcas were taken as the explants for studying the techniques of callus induction and cell suspension cultures. The result shows that the optimal medium for callus induction is MS+2,4-D0.6mg/L+ BA1.0mg/L + Sucrose 30g/L, in which the callus is humid, loose and colorful. The fine suspension cell system have been established by inoculating the callus in the medium of MS+NAA0.2mg/L+2,4-D1.0mg/L+BA0.5mg/L for 13 days of cultivation, and the rotation speed should be lower than 120rpm in the culture of oscillation. QD-labeled chitosan-DNA complexes as nano transgenic system, using CdSe as bio-labels and chitosan-DNA(CS-DNA) as nano-scale genic carriers, were prepared and shown to have uniform particle sizes and superior fluorescence properties. Confocal laser scanning microscopy(CLSM) confirmed the target DNA from QD-labeled chitosan-DNA complexes was integrated into the plant cell and suggest the possibility of stable transformation in Jatropha curcas.