Micro separation and determination of mammalian acidic glycosaminoglycans (mucopolysaccharides)

1967 
Abstract The determination of individual GAG in a mixture based on differential solubilization of the GAG-CPC complexes from a cellulose micro column (1) has been examined. The method has permitted separation of 100 μg of GAG into seven reproducible fractions which were analyzed directly for uronic acid. Analyses of 20 samples were readily completed in a day. Over-all recovery and reproducibility of each GAG fraction were within ±3 μg uronic acid. Each fraction was nominally identified with one of the eight defined mammalian GAG. The distributions of GAG in a variety of connective tissues, as analyzed by this method, were similar to those reported in the literature based on classical methods of separation. Factors that might compromise the identification of a fraction with a specific GAG were considered and studied. The application of supplementary methods to the fractions which make the characterization of the GAG more reliable was demonstrated.
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