Combinations of capillary electrophoresis-UV/Vis and molecularly imprinted polymers for detection of phytoestrogens

Phytoestrogens intake plays an important role in cancer treatment or prevention of tumor or heart disease, cardiovascular disease and others chronic diseases such as osteoporosis. Soy food is the most significant source of these phytoestrogens. The most commonly used methods for phytoestrogen determination include electrophoretic or chromatographic separation mechanics. In this study, the benefits of coupling molecularly imprinted polymers with capillary electrophoresis-UV/Vis for detection of phytoestrogens are shown. Polydopamine imprinted layer formed enables efficient isolation/extraction of target compounds (genistein and biochanin A) from milk sample and consecutive microcolumn separation with absorbance detection enables to distinguish nonspecifically bound interferents.