Byproducts from cassava industry: alternative substrates for cyclodextrin glycosyltransferase production by alkalophilic Bacillus trypoxylicola SM-02

espanolEn el presente trabajo estudiamos el uso licor de maiz fermentado (LMF), harina de cascara de yuca (HCY) y aguas residuales de yuca para la produccion de ciclodextina glicosiltransferasa (CGTase) por un nuevo aislado alcalofilo de Bacillus trypoxylicola SM-02 en fermentacion sumergida. Los experimentos se realizaron por Diseno Central Compuesto Rotativo 22 totalizando 11 ensayos. La mayor actividad enzimatica de 352.53 U/mL se obtuvo con 1.5 g de HCY y 0.6 g de LMF. La temperatura y el pH optimos fueron 55 oC y pH 8.0, respectivamente. CGTase mostro una actividad relativa superior al 50% durante 120 min. a la temperatura optima. Solo el CaCl2 mostro actividad positiva para CGTasa. Los resultados apuntaron a un buen potencial de B. trypoxylicola SM-02 para la produccion de CGTasa usando substratos residuales. EnglishIn the present work was studied the use of cassava peel flour (CPF), corn steep liquor (CSL), and cassava wastewater as substrates to produce cyclodextrin glycosyltransferase (CGTase) from a new alkalophilic isolate of Bacillus trypoxylicola SM-02 by submerged fermentation. The experiments were performed as a Central Composite Design 22 , totalizing 11 assays. An enzymatic activity of 352.53 U/mL was obtained using 1.5 g of CPF and 0.6 g of CSL. The optimum temperature and pH of CGTase was 55 °C and 8.0, respectively. The CGTase depicted a relative activity of more than 50% for 120 min at the optimum temperature. The only salt that positively influenced the CGTase activity was CaCl2. The results are indicative of a potential role of B. trypoxylicola SM-02 in the production of CGTase using residual substrates.