Effect of Cu~(2+) on the Culture of Haemocytes from Macrobrachium nipponense

Haemocytes of Macrobrachium nipponense cultured in vitro were studied. It was found that satisfactory results could be obtained using medium 199 supplemented with 15% fetal bovine serum (FBS), 100?IU/ml Penicillin, 100?μg/ml streptomycin osmolarity adjusted to 472?mmol/L. The effect of Cu 2 on subcultured haemocytes was studied. Basal medium supplemented with Cu 2 2?μg/L enhanced the growth of haemocytes and improved their total phosphate and AKP activity.