Effect of Proliferation and Invasion of Breast Cancer Cell by PP2

Objective To study whether PP2 [4-Amino-5-(4-Chloro-Phenyl)-7-(t-Butyl) Pyrazolo Pyrimidine] could change the expression of src kinase and E-cadherin protein,and detect the proliferation and invasion of breast cancer cell.Methods After treating MDA-MB-231 with PP2,western blot was performed to determine the expression of src and E-cadherin;The proliferation was been tested by MTT assay;Boyden chamber assay was used to examine the effect of the invasion of MDAMB-231 cell.Results Western blot show if the dosage of PP2 increase,the expression of src is down-regulated and E-cadherin is up-regulated.MTT assay show that the proliferation of MDA-MB-231 have association with PP2 dosage(P0.05).Boyden chamber assay show that the invasion is down-regulated when the dosage of PP2 is enhanced(P0.05).Conclusion PP2 can strongly inhibit src and enhance the expression of the downstream protein of E-cadherin.The proliferation and invasion of MDAMB-231 cells is also inhibited by PP2.