Map‑based cloning reveals the complex organization of the BnRf locus and leads to the identification of BnRf b , a male sterility gene, in Brassica napus

Zonghan Deng,Xi Li,Zengzeng Wang,Yingfen Jiang,Lili Wan,Faming Dong,Fengxiang Chen,Dengfeng Hong,Guangsheng Yang

Map‑based cloning reveals the complex organization of the BnRf locus and leads to the identification of BnRf b , a male sterility gene, in Brassica napus

2016

Zonghan Deng
Xi Li
Zengzeng Wang
Yingfen Jiang
Lili Wan
Faming Dong
Fengxiang Chen
Dengfeng Hong
Guangsheng Yang

Key message Sequencing of BAC clones reveals the complex organization of the BnRf locus and allowed us to clone BnRf b , which encodes a nucleus-localized chimeric protein BnaA7.mtHSP70-1-like.

Keywords:

clone (Java method)
Botany
Physical Chromosome Mapping
Cloning
Genetics
Locus (genetics)
Open reading frame
Biology
Allele
Gene
Bacterial artificial chromosome
Fusion protein
Sterility

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