Determination of phenolic acids and rutin in Heliotropium thermophilum by high-performance liquid chromatography with photodiode array detection

ABSTRACTA novel and rapid method was validated for the determination of phenolics in plants by high-performance liquid chromatography coupled to photodiode array detection. The optimized method was employed for the quantification of phenolic acids and rutin present in Heliotropium thermophilum. Eight samples collected from two locations were analyzed. Additionally, temperature dependent changes in the phenolic composition of H. thermophilum samples was evaluated. A series of standards were used for identification that included gallic acid, protocatechuic acid, 4-hydroxybenzoic acid, chlorogenic acid, vanillic acid, caffeic acid, syringic acid, p-coumaric acid, rutin, ferulic acid, benzoic acid, 2-hydroxycinnamic acid, abscisic acid, and trans-cinnamic acid. Chromatographic separation was performed on C8 column at 40°C with gradient elution. A phosphate buffer (20 mM, pH: 2.5) and acetonitrile mixture was used as the mobile phase with detection at 280 nm wavelength and a flow rate of 1.5 mL/min. The develo...