Analysis of Orosomucoid and C-Reactive Protein Levels in Gingival Tissue And Serum of Rats with Experimental Periodontitis: Comparison at different time points in disease progression

Objectives: Acute-phase proteins are thought to trigger periodontal tissue destruction. The core purpose of this study is to analyze the levels of Orosomucoid1 (ORM1) and C-reactive protein (CRP) in gingival tissue and serum of rats with experimental periodontitis. Material and Methods: Thirty rats were divided randomly and Group 1 was specified as control group, while the remaining groups were classified as ligature-induced experimental periodontitis groups. Each group was constituted 10 rats and Group 2 was evaluated the impact of ligature for the term of 7 days. Group 3 was classified on the basis of 14 days ligature. With the help of ELISA, gingival tissue and serum samples was utilized for measuring CRP and ORM1 levels. Results: Alveolar bone and attachment loss was statistically higher in all experimental periodontitis groups than those in control group ( P <0.001). The levels of CRP and ORM1 in gingival tissue were significantly higher in Group 2 than in Groups 1 and 3 ( P <0.05). Also, a statistically significant positive correlation was found between CRP and ORM1 levels in the gingival tissue ( P <0.001). Conclusions: The present results reveal that tissue destruction at earlier periods of inflammatory periodontal disease may be associated with ORM.