Cryopreserved chondrocytes in porous biomaterials with surface elastin and poly-L-lysine for cartilage regeneration.

Abstract The ability of cryopreserved chondrocytes to revitalize and propagate is a key biotechnology in cartilage regeneration. This study shows the formation of neocartilage from cryopreserved chondrocytes in scaffolds grafted with elastin and poly- l -lysine. Cryopreserved chondrocytes in elastin- and poly- l -lysine-grafted constructs were cultured in a dynamic bioreactor and assessed by biochemical assay and staining. Elastin demonstrated a better efficacy for recruiting cryopreserved chondrocytes onto the pore surface of constructs than poly- l -lysine. However, surface elastin and poly- l -lysine did not significantly enhance the biocompatibility to cryopreserved chondrocytes. Chondrocytes multiplied from cryopreserved chondrocytes in elastin-grafted constructs is faster than that in poly- l -lysine-grafted constructs. In addition, elastin could stimulate cryopreserved chondrocytes to synthesize more glycosaminoglycans and collagen than poly- l -lysine. Porous biomaterials with surface elastin and poly- l -lysine can maintain active chondrocytic proliferation and extracellular matrix secretion from chondrocytes with appropriate cryopreservation.