SPECTROPHOTOMETRIC METHOD FOR THE ESTIMATION OF TIZANIDINE IN BULK AND TABLET DOSAGE FORMS

A simple, sensitive spectrophotometric method has been developed for quantitative determination of Tizanidine hydrochloride in bulk and pharmaceutical formulations with application of factorial design. In this method, Tizanidine HCl reacts with Folin-Ciocalteu (FC) reagent under alkaline conditions forming a blue chromogen having absorption maximum at 663nm. Beer's law was obeyed in the concentration range of 4-36μg/ml. The results of the analysis were validated as per ICH guidelines and by recovery studies.