Peptide ligand sequence target-binding to lipase, and application thereof

The invention relates to a peptide ligand sequence target-binding to lipase, and an application thereof, and belongs to the field of polypeptide design and virus antigen detection. A peptide ligand binding to the lipase is finally obtained by searching optimum peptide ligands binding to a target protein and having optimum affinity in a virtual polypeptide database through a molecular docking technology on the basis of the crystal structure of the lipase by means of molecular docking and virtual screening technologies, and the sequence of the peptide ligand is RRRDCW, that is P19. The P19 is artificially synthesized, the P19 and the lipase undergo affinity identification, and the interaction balance dissociation constant KD between the sequence of the P19 and the artificially expressed lipase is 3.653 * 10 M, that is 36.53 nM, so the affinity is good. The synthesized P19 sequence can bind to artificially expressed proteins and artificially purified proteins, has high response to the lipase, has no cross response to other proteins, and has good specificity.