Effect of Lactobacillus plantarum LS/07 in combination with flaxseed oil on the microflora, enzymatic activity, and histological changes in the development of chemically induced precancerous growth in the rat colon

This experimental study showed the effect of L. plantarum LS/07 on the changes in total counts of coliform bacteria, enzymatic activities, cytokine and histological changes in the colonic mucosa, chemically induced by the application of 1.2-dimethylhydrazine dihydrochloride (1.2-DMH) in rats once a week during (P < 0.001) enzymatic activity of s-glucuronidase and β-glucosidase was found in PRO-O group compared with positive control group. The cytokine activity of tumor necrosis factor (TNFα) was significantly decreased in groups PRO (P < 0.001) and PRO-O (P < 0.01) compared to the positive control group. The histological exami- nation showed different changes (aberrant crypts and goblet cells) in experimental groups in comparison to the negative control group. Our study indicates that L. plantarum LS/07 in combination with flaxseed oil had a positive influence on colon microflora, enzymatic activities, and cytokine activity. The total results showed that potentiating effects of natural components were markedly achieved in the experimental group PRO-O.