The Investigation of Anti-Proliferative Effects of [Ag2(sac)2(dap)2] Complex on Different Types of Cancer

Objective: Cytotoxic features of silver and saccharine compounds have taken attention due to the fact that most of them have showed a cytotoxic effect against tumor cells. In this study, it is aimed to investigate anti-proliferative effects of [Ag2(sac)2(dap)2] complex on different types of cancer cell lines. Methods: HeLa (cervical cancer), PC-3 (prostate cancer), DU-145 (prostate cancer), A549 (non-small cell lung cancer), K562 (chronic myeloid leukemia) and MRC-5 (normal lung fibroblast) cell lines were grown in plates. In order to determine anti-proliferative effects, IC 50 values of [Ag2(sac)2(dap)2] and cisplatin on these cell lines were determined by MTT method. Results: IC 50 value of [Ag2(sac)2(dap)2] complex was 19.53 ± 1.74 μM for HeLa cell line, 17.14 ± 1.41 μM for PC-3 cell line, 18.56 ± 4.04 for DU 145 cell line, 17.93 ± 1.06 μM for A549 cell line, 3.18 ± 0.04 μM for K562 cell line and 7.25 ± 1.00 μM for MRC-5 cell line. Also, IC 50 value of cisplatin was 4.00 ± 0.47 μM for HeLa cell line, 12.29 ± 1.60 μM for PC-3 cell line, 5.05 ± 0.65 for DU 145 cell line, 12.74 ± 1.26 μM for A549 cell line, 5.90 ± 0.59 μM for K562 cell line and 5.91 ± 0.32 μM for MRC-5 cell line. Only in K562 cell line, IC 50 of [Ag2(sac)2(dap)2] complex was lower than IC 50 of cisplatin. Conclusion: Anti-proliferative activity of [Ag2(sac)2(dap)2] complex is more than cisplatin on chronic myeloid leukemia cells so this complex may be possible to be used as a treatment option especially for chronic myeloid leukemia.