Application of photosensitive model of red blood cell to evaluate the anti-photosensitive additives in traditional Chinese medicine.

With the increase of UV-sensitive groups, more and more attention is paid on the problems of UV-sensitive skins. However, the methods to evaluate the photosensitive damage are more often than not for animal testing. In this paper, a relatively less time consuming and less expensive convenient method is developed to detect the damage of UV-A by red blood cell hemolysis, first to obtain the hemoglobin of the injured red blood cell, and then to calculate the hemolytic rate. The experiments on the effects of ultraviolet light or photo sensitizer alone, the different exposure times and the different drug concentrations show that the red blood cell hemolysis does not occur under ultraviolet light radiation alone without chlorpromazine nor only with chlorpromazine without ultraviolet light radiation. With the chlorpromazine content of 0.33mg/mL in red blood cell, 50% hemolysis can be resulted after being radiated by ultraviolet for about 30min, and 100% hemolysis after 60min of radiation. 10 kinds of Chinese medicine were found with the active ingredients of antiphotosensitive from ginkgo biloba leaf, tea polyphenols, grape seed extraction, as the most potential anti-photosensitive materials. Among them, the main components of ginkgo biloba flavonoids were shown be able to prevent the damage of UV on the cell membrane from the source through the broad UV absorption capacity, tea polyphenols and grape seed polyphenols could protect the cell membrane by scavenging free radicals and other optical products.