P1-71 CARDIAC TISSUE APOPTOSIS INDUCED BY CARDIOPLEGIC SOLUTION OF CORONARY ARTERY BYPASS GRAFTING PATIENTS WITH THE COMPENSATORY EFFECT OF IGF-1 SURVIVAL SIGNALING ACTIVATION

artery disease (CAD), we seek to evaluate the association of Gly1057Asp of IRS-2 with CAD in Taiwanes. Methods: Patients receiving coronary angiography were enrolled. Mild CAD is defined as presence of significant stenosis (diameter stenosis>50%) in none or one coronary artery and severe CAD in two or three. Gly1057Asp of IRS2 are determined by polymerase chain reaction and restriction fragment length polymorphism and confirmed by DNA sequencing. Results: 481 patients were enrolled. 207 patients have severe CAD. The Gly allele frequency is 66.2% in patients with severe CAD and 54.0% in mild CAD (p<0.001). The Gly/Gly genotype frequency is 44.0% in patients with severe CAD and 31.4% in mild CAD (p<0.001). The risk of severe CAD increases according to the dosage of Gly allele(odds ratio for Gly/Asp genotype 1.944 [1.074∼3.519]; Gly/Gly genotype 3.237 [1.741∼6.019]; p for trend<0.0001) after adjustment for other risk factors. There is interaction of Gly1057Asp of IRS-2 and type 2 DM associating individuals with severe CAD. Presume the risk for severe CAD in non-diabetics with Arg/Arg genotype is 1, the risk is 1.88 (odds ratio), 0.59∼5.93 (95% confidence interval) (p=0.285) in diabetics with Arg/Arg genotype; 2.36 (1.23∼4.51)(p=0.009)in non-diabetics with Gly/Gly or Gly/Arg genotype; and 5.64 (2.76∼11.52)(p<0.001)in diabetcis with Gly/Gly or Gly/Asp. Conclusion: Gly1057 allele of IRS-2 alone or interacting with type 2 DM associates with CAD in Taiwanese.