Preservação química de cadáveres de suínos: reduzir e reciclar sem perder a qualidade no ensino

This article aims to describe the concern of the Faculty of Medicine, Universite of Sao PAulo, to reduce the number os animals used in teaching. Sexteen pigs, purchased from commercial supplier, with about 20 kilos, received pre-anesthetic, intramuscularlu, ketamine hydrochloride (15 mg/kg) and xylazine (2mg/kg), induction with thiopental sodium (10mg/kg) intravenously, marginal ear vein using 22G catheter, maintenance by infusion of 100 ml of saline containing 1g of thiopental sodium, 20ml of fentanyl 50mg/ml. Intubation, with an endotracheal tube with the aid of the laryngoscope, staying connected to the respirator. The carotid artery was cathererized for infusion of heparinized saline (2,500lU), and jugular vein eas used for circulatory system drainage. Euthanasia was performed with anesthesia overdose and supersaturated solution of potassium chloride intravenously. When the drained liquid became clear, were pefused with modified Larssen solution (100ml/kg). In parallel, we used sets chest, larynx, trachea, lungs, heart, digestive and assembliers, since the portion of the thoracic esophagus to the ilium. Both sets as whole carcasses were keptat-20oC and thawed at room temperature 48 hours before use. Carcasses kept physical characteristics. Alternative models to the animals concernet have been usef sucessfully in numerous practices in skills training of graduate studantes and residents of FMUSP, and other areas to previous studies of experimental surgery allowing the reduction recycling and reuse of swine carcasses for preserving chemical and physical. Certified CEAU 0828/08 HCFMUSP