A two-dimensional Co(II)-phosphonate framework: promotion activity on fracture via activating the wnt signaling pathway

A phosphonic acid ligand 4-(1H-imidazole-4-yl)phenylphosphonic acid (H3piz), featuring both phosphonic and imidazole donor groups, is used as a linker to construct metal-phosphonate framework [(CoHpiz)]n (1) in the mixed N,N-dimethylformamide (DMF), isopropanol, and water solvent in the presence of a small amount of hydrofluoric acid. The structural analysis results show that complex 1 has a 2D layered network which is further extended into a 3D supramolecular network via H-bond interactions. The promotion activity of the compound on fractures is then evaluated. After the model construction of fracture, cd133 (cd133 is used as a marker for hematopoietic stem and progenitor cells for somatic stem cell isolation, and it may play a role in cell differentiation, proliferation and apoptosis) is measured by real-time reverse transcription-polymerase chain reaction (RT-PCR). Then, the activation of the Wnt (Wnt is a fusion of two terms: derived from the Drosophila gene wingless (wg) and proto-oncogene integration-1 (int), which is the mammalian homolog of wg) signaling pathway in the mesenchymal stem cells is measured with the Western blot. The activity of the Co(II) complex to the fracture is further evaluated using the molecular docking simulation.