Visualization of RNA and RNA Interactions in Cells

The increasing diversity of biological pathways in which RNA molecules play a role requires different approaches to RNA studies. Among other methods, RNA studies on a single-cell level offer the unique possibility not only to measure transcription kinetics but also to obtain information on time- and space-determined RNA dynamics. The past decade has witnessed significant progress in the development of new methods allowing RNA studies on the level of single cells. These methods, and the results obtained, are discussed in this chapter. Keywords: fluorescence in-situ hybridization (FISH); proximity ligation assay (PLA); molecular beacons (MBs); aptamers; protein complementation