Point-localized, site-specific membrane potential optical recording by single fluorescent nanodiscs.
2021
Abstract Nanodisc technology was implemented as a platform for voltage nanosensors. A fluorescence (Forster) resonance energy transfer (FRET)- based voltage-sensing scheme employing fluorescent nanodiscs and the hydrophobic ion dipicrylamine was developed and utilized to optically record membrane potentials on the single-nanodisc level. Ensemble and single-nanosensor recordings were demonstrated for HEK293 cells and primary cortical neuron cells. Conjugation of nanodiscs to anti-GABAA antibodies allowed for site-specific membrane potential measurements from postsynaptic sites.
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