Comparison of chitin content in the apical and distal parts of fungal hyphae inBasidiobolus ranarum, Neurospora crassa andCoprinus sterquilinus

Primary cell wall is synthesized in the growth zone of hyphal apex in fungi and rigidified during maturation along the newly formed hypha. Cross-linking of cell-wall components and self-assembly of individual polysaccharide chains into microfibrils are supposed to be involved in the rigidification process. We determined the relative chitin content in the cell wall of hyphal tips and distal walls of three fungal species and demonstrated a general increase in relative chitin content in mature cell walls. Thus, this increase can be supposed to raise cell-wall rigidity as the principal role of chitin in the determination of cell-wall rigidity is beyond doubt.