A Synthetic Polyanion Polymer as a Growth Promoter for L929 Mouse Fibroblast

A synthetic polyanion polymer, poly(maleic acid-alt-7,12-dioxaspiro[5,6]dodec-9-ene) (MACDA), enhanced the growth of L929 mouse fibroblasts in a serum- and protein-free condition. The highest promotion efficiency was obtained at 10 μg/ml of MACDA, resulting 2.1-fold increase in the cell number against a control experiment without MACDA. At that time, no significant morphological change of L929 was observed. The unnecessary of external growth factors distinguishes MACDA from other synthetic polymers those have been known to show such a cell growth promotion.