Genetic Diversity of Rhizobia Isolated from Pueraria spp. in Sichuan,China

Twenty-three rhizobial strains isolated from Pueraria spp. in different sites of Sichuan province were studied by using AFLP, BOXAIR-PCR and 23S rDNA PCR-RFLP techniques. The results showed that genetic diversity among these rhizobia was existed. The analysis of AFLP revealed that, there were seven AFLP genotypes among these rhizobial strains; and BOXAIR-PCR suggested that, there were five genotypes existed. The results from 23S rDNA PCR-RFLP analysis revealed that, from the phylogeny point of view, these rhizobia belong to Mesorhizobium sp. and Bradyrhizobium sp., and however, most of them were different from the type strains of the related genera, which indicated that maybe there are putative new species existed.