Bupivicaine cause SH-SY5Y cells cultured by high-glucose to enhance endoplasmic reticulum stress with an increase in reactive oxygen species and apoptosis

Objective To discuss bupivacaine causing SH-SY5Y cells in high sugar environment to increase reactive oxygen species (ROS) and apoptosis.Methods we adopt 1 mmol/L bupivicaine to SH-SY5Y cells cultured by different concentrations of glucose medium,and employ flow cytometry to detect the quantity of ROS in the cells and the situation of apoptosis.we use MTT method to detect cell survival rate.At last we use western blotting technique to detect the change of the related protein about GRP78.Results Groups of cells containing different concentration of sugar medium through bupivacaine impacted 6 h have been detected that the contents of intarcellular ROS in groups of 5.6,6.1,7 mmol/L have no statistical significance (P＞0.05).Each group of cell survival rate (％) in sequence is (4.29±0.20),(3.30±0.19),6.31±0.14),(7.89±0.09),(2.81±0.12),(1.01±0.11),and cell survival rate among groups have statistical significance(P＜0.05).Each group of cell apoptosis rate(％) in sequence is (1.80±0.09),(2.82± 0.11),(3.30±0.21),(4.31±0.18),(6.28±0.13),(7.89±0.08),and cell apoptosis rate among groups have statistical significance (P＜ 0.05).Western blot detect the expression of Grp78 in group of 5.6 mmol/L and 6.1 mmol/L are (1.02±0.12) and (0.97±0.06) respectively,exceeding the group of 7.0 mmol/L.Also the difference have statistical significance (P＜0.05).The groups of 11.1 mmol/L and 13.3 mmol/L being shorter than the others and compared to the others have statistical significance (P＜0.05).Conclusions Intracellular ROS increase and apoptosis are associated with bupivacaine that further strengthen endoplasmic reticulum stress.Endoplasmic reticulum stress cause ROS to outbreak in cells,leading cells to damage and apoptos.With the function of GRP78 degrading,endoplasmic reticulum stress strengthen than ever. Key words: SH-SY5Y cell;  Reactive oxygen species;  Apoptosis;  Nerve toxicity susceptibility