MICRO-FLOW-SYSTEMS WITH BIOSENSOR DETECTION FOR IN-VIVO USE

In order to overcome the limitations of commercially available microdialysis systems we used planar micro-technologies to realise a miniaturized device comprising connection fluidics integrated to a biosensor array flow cell. In this way overcoming the need for and the risks of the assembly and also minimising the internal vohnne. The microdevice consists of a dialysis sampling system integrated to the flow-through cell of a microfabricated biosensor array for the simultaneous on-line monitoring of glucose and lactate. The fluidic connections between the different device’s components are realized by subsequent processing of stacked dry resist layers on a plastic support that provides also the means for electric connections. The performance of the device was evaluated in vitro. Recoveries of over 95% for both analytes were achieved when flow rates of the perfusion fluid 5 0.5 nllmin were used. At this flow rate, the response time of the device was 2.4 miss, which is acceptable for on-line analysis. The device showed a long-term run stability both in buffer and serum samples.