Study on Purification of Invertase from Yeast

This paper used three methods to extract invertase from yeast, which included in this manuscript, three different extraction method breaking cells by adding methylbenzene, frost grinding, and adding SDS for extracting invertase from yeast were investigated. Then the purified invertase was obtained by precipitatation with 50% ethyl alcohol and Mono Q chromatography. The molecular weight of the enzyme was 60kD by SDS-PAGE, and the pI was 5.6 by isoelectric focusing. The invertase followed typical Michaelis-Menten Kinetics with apparent Km of 0.013 mol/L. The results showed all three methods had both advantages and disadvantages. The invertase extracted by adding SDS and frost grinding had much more total activity than that of extracted by adding methylbenzene. A highest total invertase activity was found in the SDS extraction process, and it was a convenient and economical method for commerical production of invertase from yeast.