Bioremediation of Cd-spiked soil using earthworms (Eisenia fetida): Enhancement with biochar and Bacillus megatherium application.

Abstract In this study, we evaluated the influence of biochar and Bacillus megatherium on Cd removal from artificially contaminated soils using earthworms (Eisenia fetida). Within a 35-days remediation period, over 30% of Cd was removed by earthworms from the contaminated soil (with Cd at ∼ 2.5 mg kg-1), and both additives facilitated Cd removal. Additionally, over 22% reduction in the extractable Cd contents was also achieved by earthworms. Cd accumulated in earthworms steadily increased through remediation, and the accumulated Cd decreased in the order of earthworm + biochar (T3) > earthworm + Bacillus megatherium (T4) > earthworm alone (T2). The bioaccumulation factors (BCF) were above 1, indicating the enrichment of Cd in earthworms, and there were higher BCF for both T4 (944%) and T3 (845%). The ingestion of metal-bonded biochar particle and the elevated Cd mobility would be the main reason for the enhanced Cd-remediation by earthworms under T3 and T4, respectively. Through remediation, microbiota communities in both, soil and earthworm guts, demonstrated high similarity, while a lower level of bacterial abundance was observed in earthworm guts compared with that in soils. Eventually, soils became more fertile and demonstrated higher enzyme activities after remediation. Therefore, we concluded that earthworm, alone or combined with biochar or Bacillus megatherium could be an alternative method for Cd-contaminated soil remediation.