Preparation of monoclonal antibodies against HN protein of Class I Newcastle disease virus virulent strain

AIM:To prepare monoclonal antibodies (mAb) against HN protein of Class I Newcaslte disease virus.METHODS:Several 6-week-old mice were immunized with ClassⅠ 9a5b virulent strain.Hemagglutination inhibition (HI),indirect immunofluorescence assay (IFA),cell neutralization test,ELISA and Western blot analysis were used to characterize and identify these antibodies.RESULTS:Four hybridoma cell lines were successfully prepared,designated as mAb 2F5,2F12,3H7 and 3H9.Among them,only mAb 3H7 and 3H9 recognized HN protein of the ClassⅠ strain,specifically neutralizing NDV and inhibiting hemagglutination.CONCLUSION:mAb 3H7 and 3H9 could be of use in identification of Class I and Class II strains,and in functional studies of HN and cell receptors for NDV.