Test method of palmitoylated modified protein based on specific antibody

The invention belongs to the field of biochemical analysis and relates to a high-throughput test method of palmitoylated modified protein in complex biological samples based on prepared protein palmitoylated modified generic antibody serum and utilizing the same. The method includes: adopting a palmitoylated modified peptide segment as an antigen to prepare high-specificity palmitoylated modified generic antibody serum; utilizing the specific antibody serum to test protein palmitoylated modifying state. Compared with existing throughput analysis methods, the test method has the advantage of high specificity, is low in false positive rate, can directly indicate palmitoylated modified protein, can be used for samples of various sources, can identify palmitoylated modified protein in the biological samples in a high-throughput manner and is simple, convenient and easy to operate. The method provides an effective means for high-throughput testing of protein palmitoylated modifying in the biological samples.