Production of monoclonal antibodies against Streptococcus mutans antigens

Several studies have been conducted in the last decades aiming to obtain an anti-caries vaccine, however some studies have demonstrated cross reactivity between Streptococcus mutans surface antigens and the human cardiac tissue. In this work, the reactivity of five anti-Streptococcus mutans monoclonal antibodies (MoAb) (24A, 56G, C8, E8 and F6) was tested against oral streptococci, cardiac antigens and skeletal and cardiac myosins, aiming to evaluate the specificity of these MoAb. The hybrid producers of immunoglobulins of the IgG2b class were cloned by limit dilution and expanded in vivo. MoAb were tested by ELISA. The hybrid 24A reacted with S. mutans CCT 1910, S. salivarius CCT 0365 and S. pyogenes T23. No reactivity difference was observed among the tested species. Cross reactivity with heart and cardiac myosin was not confirmed and only reaction with myosin of skeletal muscle was observed (p = 0.0381). The hybrid 56G reacted with all the tested microorganisms and there was statistically significant difference between S. mutans and S. pyogenes T23 (p Diversos estudos foram realizados nas ultimas decadas com o intuito de se obter uma vacina anticarie dentaria, mas alguns trabalhos tem demonstrado reatividade cruzada entre antigenos de superficie de Streptococcus mutans e tecido cardiaco humano. Neste trabalho, foi testada a reatividade de cinco anticorpos monoclonais (AcMo) anti-Streptococcus mutans (24A, 56G, C8, E8 e F6) contra estreptococos orais, antigenos cardiacos e miosinas esqueletica e cardiaca, no intuito de avaliar a especificidade desses AcMo. Os hibridos produtores de imunoglobulinas da classe IgG2b foram clonados por diluicao limite e expandidos in vivo. Os AcMo foram testados por ELISA. O hibrido 24A reagiu com S. mutans CCT 1910, S. salivarius CCT 0365 e S. pyogenes T23. Nenhuma diferenca de reatividade foi detectada entre as especies analisadas. Reatividade cruzada com coracao e miosina cardiaca nao foi confirmada, existindo somente reacao com miosina de musculo esqueletico (p = 0,0381). O hibrido 56G reagiu com todos os microrganismos testados e houve diferenca estatisticamente significante entre S. mutans e S. pyogenes T23 (p