Predicting “Airborne” Influenza Viruses: (Trans-) mission Impossible?

Repeated transmission of animal influenza viruses to humans has prompted investigation of the viral, host, and environmental factors responsible for transmission via aerosols or respiratory droplets. How do we determine — out of thousands of influenza virus isolates collected in animal surveillance studies each year — which viruses have the potential to become ‘airborne’, and hence pose a pandemic threat? Here, using knowledge from pandemic, zoonotic and epidemic viruses, we postulate that the minimal requirements for efficient transmission of an animal influenza virus between humans are: efficient virus attachment to (upper) respiratory tissues, replication to high titers in these tissues, and release and aerosolization of single virus particles. Investigating ‘airborne’ transmission of influenza viruses is key to understand — and predict — influenza pandemics.