Effects of mesangial cells on expressions of collagen I and cbfα1 in osteoblasts base on the foundation of tonifying kidney herbs

Objective: Tonifying kidney herbs can promote the proliferation,differentiation and other functions of osteoblast.There is a intimate connection between kidney and bone.Renal mesangial cells may secrete some factors effecting osteoblast.Aim of our study is to investigate the effects of mesangial cells on osteoblast which have been regulated by tonifying kidney herbs.Methods: Rat were divided into 2 groups: control group,drenched with distilled Water;Radix Rehmanniae Praeparata(RRP) Carapax Trionycis(CT) group,with RRP CT extractum,3.3g/kg/d,for 3 days,when non-herb and RRP CT serum obtained.Osteoblasts were treated respectively with non-herb serum,contained herbs serum and contained herbs serum processed by mesangial cells for 3 days.MTT method was used to investigate proliferation rate of osteoblasts.Expressions of collagen I and cbfα1 were examined using real-time fluorescence quantity assay.Results: Contained Rehmannia glutinosa and Turtle shell serum promoted the proliferation of osteoblasts,while contained herbs serum processed by mesangial cells got further effects(P0.05).Results of real-time fluorescence quantity assay showed that expressions of collagen I and cbfα1 were upregulated in contained herbs serum group(P0.05) and the effects were reinforced by mesangial cells(P0.05).Conclusion: Mesangial cells can further promote proliferation of osteoblasts and expressions of related genes.