Fractionation and Identification of Some Phenolics Extracted from Evening Primrose Seed Meal

Crude ethanol and ethyl acetate extracts of evening primrose (Oenothera biennis L.) seed meal were separated into fractions using Silicagel L 100/200 column chromatography and methanol as the mobile phase. Six fractions were obtained from the ethanol extract and five fractions from the ethyl acetate extract and their contents of total phenolics were determined. By means of HPLC, the active compounds were identified as catechin, gallic acid, caffeic acid, syringic acid, and ferulic acid.