The method of extracting the whole truth stretch tang polysaccharide purified

The present invention discloses a completely true stretch extracting one polysaccharide purification of soup, Chinese medicine extraction technical field belongs, to a method for simple, extraction and purification of the polysaccharide content; the method is to weigh the components, except Rehmannia remaining outside the drying pulverized herbs; Rehmannia will cut into sections, and extracted with ultrasonic extraction, the supernatant was centrifuged; the supernatant was concentrated, adjusted to an ethanol content of 75-85% liquid, standing and centrifugation, the lower layer A precipitate was dried to give crude polysaccharides; taking A crude polysaccharide dissolved in water, shake, an acid to adjust the pH 5-6, papain was added, enzyme inactivation precipitate was removed by centrifugation, the lower layer was taken to obtain a crude enzyme solution A polysaccharide; the a liquid polysaccharides enzyme added chloroform - n-butanol solution, shock, allowed to stand overnight, the upper aqueous phase solution was collected to obtain the polysaccharide solution a; formulated as aqueous solution was added to adjust the pH of the polysaccharide solution to 4.5-5.5, macroporous resin adsorption, filtration to give crude polysaccharides, dialysis bags into a dialysis solution to obtain purified polysaccharide.