Efficiency of three methods for detecting Toxoplasma IgG antibody

Objective To discuss the test efficiency of three methods for detecting Toxoplasma IgG antibody. Methods Totally 304 specimens were detected parallelly for Toxoplasma IgG antibody by using the gold marked method,indirect hemagglutination test(IHA),and enzyme-linked immunosorbent assay(ELISA),and the sensitivity,specificity and Youden index of these methods were compared. Results The detection sensitivities of gold marked method,IHA,and ELISA for Toxoplasma IgG antibody were 85.5%,89.8% and 91.9% respectively(χ2= 4.12,P 0.05);the specificities were 92.4%,96.6% and 97.5% respectively(χ2= 4.06,P 0.05). The detection efficiency and Youden index of ELISA were 94.1% and 0.89 respectively,being higher than those of IHA and gold marked method. Conclusion The sensitivity and specificity of the ELISA method for Toxoplasma IgG antibody are higher,and in addition,it can be automated. Therefore,it is suitable for large-scale Toxoplasma IgG antibody screening.