Enzymes, receptors, and carriers of biological membranes : a laboratory manual

I. Analytical Separation Techniques.- Electrophoretic Transfer of Chloroplast Membrane Proteins from SDS-Gels onto Nitrocellulose and Their Immunological Detection.- Investigation of Amphiphilic Nature of Different Forms of Acetylcholinesterase from Torpedo Marmorata by Charge Shift Crossed Immunoelectrophoresis.- Separation of Hydrophobic Membrane Proteins by Phase Partition: Characterization by Two-Dimensional Gel Electrophoresis and Silver-Staining.- Quantitative Analysis of Plant Membrane Lipids by a Combined TLC-GLC Procedure.- Localization of Phosphatidylglycerol in the Membrane of Acholeplasma laidlawii.- II. Protein Structure and Interactions.- Crystallization of Two Membrane Proteins: Bacteriorhodopsin and Photosynthetic Reaction Centres.- Investigation by Crossed Immunoelectrophoresis of Membrane-Cytoskeleton Interactions in Human Erythrocyte Membranes.- III. Labeling and Crosslinking.- Labeling of the Hydrophobic Core of Membranes with 3-trifluoromethyl-3-(m-[125I] iodophenyl) diazirine: Measurement of the Time-Course of the Photolabeling Process.- Selective Labeling of the ADP/ATP Translocator with Eosin-5-Maleimide.- Site-Directed Hydrophobic Labeling of Membrane Proteins: NBD-Modification of Bacteriorhodopsin.- IV. Reconstitution.- Reconstitution of Na, K-ATPase.- Import of Proteins by Isolated Mitochondria.- Isolation and Functional Reconstitution of Rat Liver Cytochrome Oxidase.- V. Spectral Techniques.- Fluorescent Labeling of Band 3 Protein from Erythrocytes.- Spin Labeling of Membranes and Membrane Proteins.- Spectroscopic Measurements in the Subsecond to Second Time Domains: Use of an Optical Multichannel Analyzer for Spectral and Temporal Data Acquisition.- Use of a Potential Sensitive Dye in Studies on Phospholipid and Sarcoplasmic Reticulum Vesicles.- VI. Receptors.- Beta-Adrenoceptor Regulation in Organs of Rats Chronically Treated with Reserpine or Isoproterenol. Radioligand-Receptor Binding Studies.- Functional Changes in the Beta-Adrenoceptor Adenylate Cyclase System During Reticulocyte Maturation in vitro.- Acetylcholine Receptor-Enriched Membrane Fragments from the Electric Organ of Torpedo Marmorata.