495 Maternal and Endogenous Iga Protection in Infants with Respiratory Tract Infections

Background Intestinal Ig A protection in infants relies both on maternal human milk sIgA (secretory immunoglobulin A) controlled by chemokines like CCL28, with roles of amplifying mammary secretion and passive gut epithelium transfer, and endogenous production. Probiotic strains from human milk and their substrate oligosaccharides derived from lactosis may stimulate local Ig A production in infants. Objectives Assessing the levels of IgA in human milk and in infant’s serum and lactosis levels from human milk, in breastfed infants with respiratory tract infections. Methods We have evaluated 40 pairs mother- infant, healthy mothers, infants with respiratory tract infections. Human milk samples were analysed for physical and chemical properties on an ultrasonic infrared spectrometric analyser (ph, temperature, density, conductivity, fat composition, lactosis levels). Ig A, Ig M, IgG levels and protein profiles from human milk were measured after centrifugation by immunoturbidimetry method on a spectrophotometer and by protein electrophoresis with celullose acetate membrane respectively. Serum Ig A, Ig M, IgG levels from infants were determined using the same immunoturbidimetry method. Pearson correlations were studied in accordance to study’s objectives. Results Positive correlations statistically significant (p Conclusions Serum levels of immunoglobulins in infants (both IgA and IgG) seems to be more related to human milk IgG and lactosis levels rather than human milk IgA and their specific chemokines.