New Fluorescent Strategies Shine Light on the Evolving Concept of GPCR Oligomerization

GPCR oligomerization has been a matter of intense research these last years. FRET and BRET methods have paved the way to a generalized concept of potential GPCR oligomerization in artificial systems (transfected cell lines). More recently, the use of fluorescent ligands compatible with time-resolved FRET studies has opened the possibility of GPCR oligomerization study in their native context and brought evidence of their existence. Furthermore, recent applications of original fluorescence techniques are unveiling new information on the dynamics that govern these complexes and are changing the way we see GPCR oligomeric structures.